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We used a FRET-based approach to show that PKCs phosphotyrosine-binding C2 domain interacts with the Tyr 311 -phosphorylated V3 region ( Figure 4

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Fitting these data to the Hill equation gave an apparent affinity for Na + in the low mM range (8.62 1.8 mM) (Supplementary Table 3), in line with previous studies 6,22,43,44 , with one report indicating a lower apparent K M of 0.3 mM 23 , which may reflect the choice of N -methyl-d-glucamine as a counter-ion versus Cs +

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