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Intracellular GPX4 activity detection Initially, EJ cells (5 10 5 ) were cultured in 6-well plate with a CO 2 incubator overnight at 37 C

Estimation of lipid peroxidation Cells were incubated with the fluorescent probe C11-BODIPY (10 m, D3861, Invitrogen, USA) for 30 min according to the manufacturers protocols

Guay, C., Madiraju, S

Antioxidants 10(2):201 Wang X, Zhang F, Liu Z, Feng H, Yu ZB, Lu Y, Zhai H, Bai F, Shi Y, Lan M, Jin J (2008) Effects of essential oil from Croton tiglium L

(2016) also measured the increasing trend of antioxidant activity during fermentation process of milk kefir using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay, a test used to quantify a substances ability to neutralise free radicals

68(6): 598604 Krishnaswamy K, Kalamegham R, Naidu NA