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C559V, C578S, C619I, and C813A in GluK5) were incorporated, the C-terminal region of GluK5 after the M4 helix was truncated after residue 829, and the GluA2 tail (YKSRAEAKRMK) was added to the C-terminal of GluK5 to promote subunit expression and heteromer monodispersity

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