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The mixture should be crumbly and moist, not dry

Immunofluorescence and microscopy Frozen brain tissue sections (8 m thickness) in glass slides were washed with PBS, fixed in acetone-methanol (11 v/v) fixative, blocked the cellular antigen with 3% bovine serum albumin at room temperature for 1 hr in the presence of 0.4% Triton X-100 and incubated with respective primary antibodies such as mouse anti-cPT1/ant-cPT2 (1250 dilution) and rabbit anti-neurofilament (1250 dilution) for overnight at 4C

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