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doi:10.1007/s12011-023-03845-3 Gao PC, Chu JH, Chen XW, Li LX, Fan RF

Immunofluorescence confocal microscopy After the indicated treatment, EGFP-NINJ1-overexpressing HT-1080 cells, with or without mCherry-xCT or TFRC-mApple overexpression, were fixed with 3.7% formaldehyde, permeabilized using 0.3% Triton X-100, and blocked with 5% BSA for 1 h

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If the ability to reduce GSSG to GSH is overwhelmed by a high burden of oxidative stress, GSSG can be exported out of cells or react with protein sulphydryl groups to prevent a major shift in the redox equilibrium [11], thereby depleting cellular GSH