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Specific investigations include the Ham's test which exposes erythrocytes to a solution at pH 6.4: normal erythrocytes can handle this but those of paroxysmal nocturnal haemoglobinuria cannot

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On the other hand, these transition metals are highly reactive and, when present in excess, lead to the accumulation of reactive oxygen species (ROS) and oxidative damage to cellular components (Halliwell, 2006)

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