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Chromatographic Separation: A reverse-phase C18 column with a gradient elution profile was used to separate GSH and GSSG from other metabolites
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Samples were then centrifuged at 15,000 g at 4 C for 20 min
[38] found that in both PASMC and PAH rats, HIF-1 regulated mitochondrial dynamics during hypoxia-induced pulmonary vasculature remodeling by directly lowering Drp1 expression and increasing the expression of Mfn2 (Figure 1)
