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Store the lyophilised powder at -20C, kept dry and protected from light

Next, pyruvate is converted into fermentation products such as lactate, ethanol, and organic compounds, including acetate, butyrate, and propionate, in which two NAD + molecules are generated, fed back, and reutilized in the glycolysis process 75

Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

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Enzymes involved in tryptophan metabolism are studied as potential therapeutic targets

The interface formed between the Rhod and PDO domains is rather limited, largely involving residues 290-296 of the PDO domain and residues 415-425 of the Rhod domain within the same subunit (Fig