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Transfection of overexpressing or siRNA plasmids were carried out in 6070% confluent cells by using Lipofectamine 3000 reagent according to the manufacturers protocol

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For non-normally distributed data, we used the Mann Whitney U test for comparing two groups, Kruskall-Wallis or Friedman test, with Dunn's multiple comparisons as a Post Hoc analysis, as well as Scheirer-Ray Test, a non-parametric alternative to multi-factorial ANOVA

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For instance, when Yao et al

Reduced lung-cancer mortality with low-dose computed tomographic screening