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For Syn 1103 and Syn 1103 -104C constructs, eluted fractions were further purified using a HiTrap Heparin HP column (Cytiva, MA, USA), leveraging cation-exchange conditions due to their higher isoelectric point (pI ~9.5)

Development of a Novel Assay for Direct Assessment of Selective Amylin Receptor Activation Reveals Novel Differences in Behavior of Selective and Nonselective Peptide Agonists

They are also inactivated by other processes even while ligand is still present

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