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5c, intensity profiles of the individual units in microtubules, F-actin and tubulin spirals were plotted using the line scan-plot profile option in ImageJ, and the intermolecular spacing was measured as the distance between the corresponding peaks of a given line scan

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The animals were immobilized with a dart gun 39 and a sample of blood was drawn from the jugular vein into EDTA-tubes (S-Monovette K3E, Sarstedt, Hildesheim, Germany) approximately 1530 minutes after darting

In contrast, the titration of acetic acid will give very different results depending on whether methyl red or phenolphthalein is used as the indicator