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This mechanism of eNOS uncoupling is unique in that it occurs in the reductase domain, unlike other uncoupling mechanisms that occur in the oxygenase domain, and can be inhibited by L-NAME (Chen et al., 2010)

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The theory is that the bodys signaling pathways reach their limit at low concentrations regardless of body size

DOI: 10.1159/000502482 50

One explanation of the difference between the effects of overexpressing monofunctional -ECS and bifunctional -ECS/ GSH-S in the tobacco chloroplast is that lesions are caused by -EC accumulation
