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Description
Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)

Conversely, adding 1.2 mL instead of 1.0 mL dilutes to 8.33 mg/mL, and every dose is about 17% weaker than planned

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Administration of NAD + precursors, such as nicotinamide riboside (NR), has been shown to reduce microglial activation, enhance mitochondrial function, and promote neuronal survival (Khoury et al., 2018)

10.1289/ehp.95103608 Environ

doi: 10.34172/apb.2024.021 [DOI] [PMC free article] [PubMed] [Google Scholar] 138.Tang DL, Chen X, Kang R, Kroemer G
