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The reaction mixture for each well consisted of 5 L of master mix, 0.5 L of each forward and reverse primer (10 M) and 1 L of 1 in 10 dilution of cDNA

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100,101 In addition, populations such as the Navajo Nation and Tibetans who live at high altitudes exhibit increased risk of developing CKD independent of glycemic control and lipidemia status

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Furthermore, this review aspires to bridge existing knowledge gaps by integrating biological, cultural and environmental dimensions, thereby providing a comprehensive and holistic understanding of this multifaceted coaction