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After 24 h (for DPBS containing glucose and MG) or 48 h (for BEGM or RPMI1640) incubation at 37 C, CCK-8 assay was performed by adding 10 L CCK-8 solution (Cell Counting Kit-8, Donjin) to each well
Specifically, the binding of IGFBP3 to GRP78 increases autophagic site formation and autophagic system flux, thereby promoting breast cancer cell survival even under glucose starvation and hypoxic conditions (57)
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Recruitment of neutrophils and NK cells to the lung parenchyma is mediated by the chemokines, including CCL2, CCL5, CXCL8, and CXCL10

Mammals, in particular, have developed biochemical and metabolic pathways to control pathogen infection by increasing amino acid catabolism to aid immune responses, thus restricting the availability of nitrogen-containing nutrients to invading microorganisms [1]
