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This guide breaks down both options clearly so you can make the right call

Arab J Biotech 12:149174 Zhang J, Li W, Xiang T, Liu Z, Laluk K, Ding X (2010) Receptor like cytoplasmic kinases integrate signaling from multiple plant immune receptors and are targeted by a Pseudomonas syringae effector
& Conesa, A

One round of 3D classification with 4-binned images and a model from a subset of the data was used to remove bad particles and contamination

Kaltunas J, et al

A central player in this process is glutathione peroxidase 4 (GPX4), an enzyme responsible for neutralizing lipid peroxides
