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This may be due to the rapid metabolization of GSH during the collection, freezing, and fixation interval, as demonstrated using mouse brain (Miller et al., 2009)

Age 36:15 Luo F, Sandhu AF, Rungratanawanich W, Williams GE, Akbar M, Zhou S, Song BJ, Wang X (2020) Melatonin and autophagy in aging-related neurodegenerative diseases

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[DOI] [PubMed] [Google Scholar] 31.Sarris J., Byrne G.J., Bousman C., Stough C., Murphy J., MacDonald P., Adams L., Nazareth S., Oliver G., Cribb L., et al

Various conditions are responsible for B 12 deficiency