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Further, our analyses revealed that the multi-omics signature associated with higher circulating TMAO concentrations and, therefore, elevated cardiovascular risk, shifted from obesity with increased insulin resistance toward overt T2D and IHD with only three variables (age, eGFR, and serum butyryl-carnitine) consistently being strong contributors across models built with the BMIS, T2D and IHD disease groups

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2.5 Determination of protein concentration The concentration of purified proteins was determined using Bradford reagent (Sigma-Aldrich) according to the manufacturers instructions with bovine serum albumin as a standard

Biotin is found in many foods, but the assessment of its dietary intake is hampered by the absence of biotin in nutrient composition databases