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The stained sections, indicative of various cellular and tissue elements, were systematically analyzed under a bright-field microscope, with images captured for detailed qualitative and quantitative assessment of histological characteristics and specific protein expression patterns 43
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Calibration was performed using standard concentrations of vitamin B 12 (20-160 g/ml) in the presence of constant concentrations of vitamin B 1 and B 6 (each 0.02 mg/ml)
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