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Partial oxygenation results in a Hb conformational change that produces a dramatic increase in the rate of autoxidation and the affinity of Hb for the RBC membrane (Cao et al., Extracellular hemoglobin as a source for oxidative stress Extracellular Hb, whether originating from hemolysis of RBCs or the infusion of cell-free Hb-based blood substitutes, can be a major source for oxidative stress

For mouse serum collection, the animals were anaesthetized with isoflurane, and blood was collected via the retro-orbital venous sinus into BD microtainer tubes (BD, 365967)
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Similar antioxidant effects have also been reported with studies using beetroot juice

Advancing Predictive Hepatotoxicity at the Intersection of Experimental, in Silico, and Artificial Intelligence Technologies
