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RNA isolation For RNA sequencing, total RNA from primary ovarian cancer cell cultures, SKOV3 cells, and fibroblasts was isolated using the RNeasy Mini Kit (Qiagen, #74104)

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Phase 2 data published in June showed that once-weekly CagriSema led to an average 15.6% bodyweight reduction at week 32 among type 2 diabetes patients living with overweight or obesity

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A similar approach was successfully applied to develop selective drug activators of the protein kinase AMPK, which were revealed to bind to an unexpected allosteric site on AMPK that is distinct from the canonical AMP-binding sites [213,214,215,216]

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