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Each cell pellet was lysed in 10 mL chromatography buffer A by sonication, and 0.5 mL aliquots of the cleared lysate were applied per well on top of the -CD affinity matrix (ten wells with AmpC-GG-Pap and three wells each with untagged AmpC and the lysate from untransformed NEB Express(lowRF1))

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Mandatory pre-treatment screening includes tuberculosis testing, hepatitis panels, and baseline blood work
Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma

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doi: 10.1016/j.biochi.2006.04.018
