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Continuous perfusion of an extracellular solution (150 mM NaCl, 10 mM HEPES, 2.8 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM glucose, pH 7.3, and osmolarity of 295300 mOsm/L) was performed at a flow rate of 0.2 ml/min, accompanied by recording

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