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This issue has been extensively addressed in recent years, without a clear resolution, and we refer the reader to recent reviews on the topic [1, 2]

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Int J Mol Sci 17(12):2124

Aquatic therapy in stroke rehabilitation: systematic review and meta-analysis

Terminal dUTP nick end-labeling (TUNEL) assay TUNEL assay was implemented to identify DNA fragmentation by using the in situ cell detection kit (Roche, Germany) in accordance with the manufacturers protocol

Corticosteroids concerns: Oral or injected corticosteroids (prednisone, cortisone) can impair healing and cause bone loss with prolonged use
