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this rules out the non-specific effects of confounders in HFD-fed mice

[7] Sigma-Aldrich catalog index for 5-Amino-1-methylquinolinium iodide (lists CAS 42464-96-0)

Because injections skip this step, the absorption rate of B12 is significantly higher and more predictable than oral supplements

As expected by the increased striatal DA, pathways signifying activation of signalling by G-protein-coupled receptors, transmission across chemical synapses and calcium signalling were all positively enriched at 3 months in iSPN + mut (Supplementary Table 1a), whereas genes associated with metabolic pathways such as oxidative phosphorylation (OxPhos) and respiratory electron transport ATP synthesis were significantly downregulated in iSPN + mut compared to iSPN+wt at 3 months (Fig

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Cells were manually scraped, spun at a 100 g for 5 min, the pellets washed with PBS and then homogenized in a Tris salt buffer, transferred to ultracentrifuge tubes and spun at 163, 200 g and 4 C for 30 min to extract the soluble fraction