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With the eight-zone rotation system described in this guide (four zones per thigh, alternating legs), each spot gets 7 weeks of rest between injections

Reactive oxygen species link gene regulatory networks during Arabidopsis root development

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53 It has normal catalytic properties and does not cause hemolysis (Class IV)

Fragments per kilobase of exon per million reads mapped were calculated for each gene using Cufflinks, and read counts for each gene were obtained using htseq-count