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For both targets, the assay was evaluated over a concentration range of 0.313 to 320 M and was demonstrated to have a quantitative dynamic range spanning nearly three orders of magnitude, with lower limits of quantification being 0.330 M for GSH and 0.370 M for cysteine

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Affinity chromatography is a type of liquid chromatography, which utilizes the reversible biological interaction (affinity) between components in the mobile phase and a solid stationary phase for separation.