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Molecular analysis of the massive GSH transport mechanism mediated by the human Multidrug Resistant Protein 1/ABCC1

Brain repair and BBB permeability are strongly linked to lesion volume and stroke severity, as well as to neuroinflammation characterized by activated microglia and inflammatory cytokines[52]

Analytical FcRn affinity chromatography Analytical FcRn affinity chromatography was performed using a human FcRn Affinity Column 75 (Roche), coupled to an KTA avant 25 instrument (Cytiva)

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To address the reactivities of the redox-sensitive cysteinyl residues, we also analyzed the recombinant monothiol roGFP2 variants roGFP2 C151S and roGFP2 C208S and detected small but reproducible glutathionylation-dependent changes of fluorescence at both excitation wavelengths for roGFP2 C151S (SSG) and roGFP2 C208S (SSG) (Fig