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One important consideration is that any virulence determinant should fulfill molecular Kochs postulates (Falkow, 1988), which essentially require that (i) a phenotype or gene be specific to pathogenic members of a genus, (ii) inactivation of a gene results in mutant with reduced infectivity (the ability to cause infection) or pathogenicity (the ability to cause disease), and (iii) complementation of the mutant restores the properties of the wild-type

88 KCs are a significant source of Wnt proteins, which stimulate -catenin in hepatocytes in a paracrine manner following PHx

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10.1016/j.freeradbiomed.2013.06.029 Free Radic