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Cell 57 , 397407 (2015)

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Research on Cell Models The use of BPC-157 in various in vitro cell models has been described in the scientific literature: Fibroblasts research on cell migration and proliferation Endothelial cells research on angiogenesis processes in vitro Enterocytes research on the function of the intestinal barrier in cellular models Myoblasts research on the differentiation of muscle cells Limitations of In Vitro Tests Please note that: The results of in vitro tests do not translate directly into effects in living organisms Laboratory conditions differ significantly from biological environments Further research is necessary to fully understand the molecular mechanisms Applications in Scientific Research BPC-157 is used as a research tool in many scientific fields

Alexa Fluor-488-labelled goat anti-mouse IgG (A-11001, 1:1,000 for IF) and goat anti-rabbit IgG (A-11008, 1:1,000 for IF) secondary antibodies were from ThermoFisher Scientific

Furthermore, we treated dividing and senescent cells with 20 M of CPP-CAND and assessed caspase 3/7 activity to examine whether the apoptosis observed was caspase-dependent