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The mobile phase comprised (buffer A) water + 0.1% (v/v) trifluoroacetic acid (TFA) and (buffer B) acetonitrile + 0.5% (v/v) water + 0.1% (v/v) TFA

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Cell (1993) 75(1):7382.10.1016/S0092-8674(05)80085-6 306 LiuJPBakerJPerkinsASRobertsonEJEfstratiadisA

Mid-protocol assessment and adjustments 4-6 week evaluation: Assess progress against baseline measurements

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Hyaluronidase activity and AQP2/creatinine ratio were measured in urine every 60 minutes after water loading test by turbidimetric assay and ELISA, respectively