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In their research, they applied the Pavia Protocol consisting of 150 mg/kg intravenous NAC followed by 300 mg/kg/day until 48 h after poisoning, alongside forced diuresis until negative urinary amanitin levels were obtained

While SREBP1 controls fatty acid, triacylglycerol and phospholipid synthesis, SREBP2 regulates cholesterol generation 90

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Finally, membranes were washed and incubated with chemiluminescence reagents (ECL