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The solution may look normal at first but develop problems as it warms

Next, the sections and cells were incubated with an Alexa Fluor 594/647 donkey anti-mouse/rabbit secondary antibody (1:1000, Abcam, United States) for 1 h at 37C

Studies indicate that cells will make a glycolytic switch when NAD + demand is high and exceeds ATP demand, therefore skewing the NAD + use for this pathway 341

Vehicle controls matched to the combined reconstitution solvent are required for baseline comparison

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