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Western Blot Western blotting was carried out as described previously [21,22], and brain homogenates were lysed on ice for 30 min in 100 mL of lysis buffer (120 mM NaCl, 40 mM Tris (pH 8), and 0.1% NP40) and centrifuged at 12,000 rpm for 30 min

Peptides function as signaling molecules in the body, helping to regulate processes such as healing, muscle building, and metabolism

Research indicates that the timing and duration of growth hormone exposure may be critical for optimizing bone and connective tissue benefits, with pulsatile patterns potentially being more effective than continuous exposure for promoting balanced remodeling and avoiding potential adverse effects such as excessive bone turnover or joint swelling

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The laboratory animals were housed in polycarbonate (PC) cages in conventional laboratory conditions at 2024 C, a relative humidity of 4070%, and a noise level of 60 dB