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Fragment 176-191 , as the name implies, is a small fragment of the growth hormone (hGH), also referred to as the fat-burning peptide for its potential in that area

Abbreviations 7nAChRs: Alpha-7-nicotinic acetylcholine receptors A: Amyloid- AD: Alzheimers disease ASD: Autism spectrum disorder ACh: Acetylcholine Akt: Protein kinase-B Bax: Bcl-2-associated X protein Bcl-2: B-cell lymphoma-2 CNS: Central nervous system DhE: Dihydro--erythroidine GABA: Gamma-aminobutyric acid GSH: Glutathione GPX: Glutathione peroxidase Hg: Mercury IL-1: Interleukin-1 MDA: Malondialdehyde MLA: Methyllycaconitine NF-B: Nuclear factor kappa B Nfr2: Nuclear factor erythroid 2related factor 2 NO: Nitric oxide NDDs: Neurodevelopmental disorders NMDA: N -methyl-D-aspartate PI3K: Phosphoinositide 3-kinases PV: Parvalbumin ROS: Reactive oxygen species TNF-: Tumor necrosis factor-alpha WHO: World Health Organization References Hodges H, Fealko C, Soares N (2019) Autism spectrum disorder: definition, epidemiology, causes, and clinical evaluation

Common side effects include mild pain at the injection site, dizziness, or nausea

doi: 10.1371/journal.pone.0258154 4

Alkaline phosphatase of the brush border of the intestines, which participates in thiamine transport (Figure 2), plays protective roles against pathogenic infection (191) and bacterial toxins (LPS) in the gut, and may counteract inflammation (192)

After polynucleotide kinase treatment, the annealed oligonucleotides were ligated into the digested vector