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Deformity or angle of bending was measured as the angle of deflection of the laser beam that was refracted from a small mirror fixed at the end of the bone

The subcutaneous (SubQ or SQ) tissue, also known as the hypodermis, is the innermost (deepest) layer of skin

This is followed by multiple immune abnormalities: marked impairment of the immunosuppressive function of myeloid-derived suppressor cells (MDSCs), pro-inflammatory polarization of Kupffer cells, T-cell differentiation imbalance, and B-cell hyperactivation that induces antimitochondrial antibody (AMA)-mediated cytotoxicity

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To investigate the involvement of the vB12 pathway in the metabolic phenotype, we treated Tbx1 +/ and Df1/ + mice postnatally with vB12 or PBS (vehicle) twice a week for 4 wk starting at 4 wk of age, after which the animals were euthanized and the brains collected