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The Atg4 protease cleaves proLC3, resulting in the formation of LC3-I

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Although one mole of ATP is used by hexokinase and an additional mole of ATP by phosphofructokinase, the net gain is 2 moles of ATP per mole of glucose, because a total of 4 moles of ATP is formed per mole of glucose by phosphoglycerate kinase and pyruvate kinase