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Representative tissue specimens (i.e., the brain, liver, kidney, pancreas, stomach, small and large intestine, lungs, and heart taken at the end of the experiment, fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h) were embedded in paraffin, sectioned at 4 m, and stained with hemalaun and eosin (H&E)

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EMJ 5 , 94103 (2020)

In addition, heterogeneity may be further introduced by the use of different assays for ferroptosis (e.g., GPX4 activity, levels of lipid peroxidation, MDA assays) and by variations in endpoint definitions