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Although drug sensitivity assays are straightforward and well-optimized once models are established, patient-derived cell lines still face challenges in becoming functional brain cancer models

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Owing to its irreversible nature, protein degradation is a highly selective process to avoid irreparable breakdown of cellular constituents, thereby disrupting cellular stability, integrity and signalling

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Further research is needed to determine (1) how many functionally distinct astrocyte-subsets exist, (2) which factors induce their differentiation, (3) how the underlying transcriptional programs relate to the differential secretion of astrocyte-derived mediators, and (4) whether these transcriptional subsets also correlate with different secretional and functional astrocyte subpopulations