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The metal boot was kept on all times until euthanasia, except during skin inspection, when rats were sedated with isoflurane and the boot was open

Beier and G

Not for use in diagnostic, therapeutic, or human or animal in-vivo procedures

We show that zonulin is a central factor in this process as it disrupts intestinal TJ proteins, enhances intestinal permeability and leads to Th1 and Th17 infiltration in the lamina propria before the onset of murine and human arthritis

doi:10.3390/ijms19071987 6

These symptoms may indicate a severe allergic reaction (anaphylaxis), which can be life-threatening if not treated promptly