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( E J ) Primary RTECs from Rest fl/fl mice were infected with control or Ad-Cre-GFP and cotransfected with control or siRNAs against GCLM ( siGCLM ) ( E ), and then subjected to HR injury for analysis of GCL activity ( F ), Western blot analyses of ferroptosis-related proteins REST, GCLM, GPX4, ACSL4, GCLC, FSP1, and DHFR ( G ), GSH levels ( H ), MDA levels ( I ), and TEM observation of ferroptosis ( J ) ( n = 3)

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[PubMed ID: 37906348]

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