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RNA extraction and RT-qPCR Total RNA was extracted from each sucrose gradient fraction using Trizol reagent (Invitrogen)
According to recent reports, SIRT1 contributes to cellular lifespan extension, resistance to oxidative stress, and repair of DNA damage (Elshazly et al
Neurother 18 , 17101728
The glutamine-alpha-ketoglutarate (AKG) metabolism and its nutritional implications
Schmidt, S
This effect was particularly strong when both drugs were given simultaneously or sequentially, i.e., 4 h apart
