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doi: 10.1016/j.bpg.2010.08.005

The transfection protocol involved seeding NPCs in 6-well plates to 6070% confluence, then incubating the cells for 6 h with a transfection complex comprising 50 nM siRNA, Opti-MEM, and Lipofectamine 2,000 followed by replacing the medium with growth medium and culturing for 72 h

The rapid shift in climate regimes causes many species to move from their area of suitable climatology to another geographic location

We set out to explore E3 ligase regulatory mechanisms through studies of the multiprotein GID complex

Physiol 293, H333H342

Depending on the clinical disease score and body weight, mice were sacrificed during the pre-acute, acute, remitting, and relapsing phase (Fig