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The occurrence of transformed Type III foci, characterized by deep basophilic staining, random cell orientation, dense multilayering of cells, and invasion into the surrounding contact-inhibited monolayer, was assessed using an optical microscope ( 2.3.3 Transcriptomics experiment 2.3.3.1 Total RNA extraction Cells in the logarithmic phase of growth were seeded at a density of 1 10 4 cells per 60 mm diameter dish using the CTA culture protocol

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