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Their results were consistent with previous data, so that corticosteroids and anticoagulants were associated with better results
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The procedure was as follows: equilibration of the affinity gel as a batch using a glass filter funnel with binding/washing buffer consisting of 10 mM TrisHCl pH 7.8, 0.2 M NaCl, 0.2 mM dithiothreitol (DTT) and 1 mM EDTA
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