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In addition to a medium made of 0.5 ml of DMEM, 10% FBS, 100 U/mL of penicillin, and 100 mg/mL of streptomycin, BPC 157 was added to each well in concentrations of 0 (control group), 0.1, 0.25, and 0.5 ug/mL for 1, 2, and 3 days in a humidified atmosphere

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However, the protocol was developed a priori and followed a standardized framework

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Lysosomal instability and cathepsin B release during acetaminophen hepatotoxicity

[DOI] [PMC free article] [PubMed] [Google Scholar] 8.Royea J., Martinot P., Hamel E