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After sedimentation supernatant was removed and crypts eluted in 10 ml PBS by shaking vigorously and passing crypts through a 70 m cell strainer to obtain Fraction 1

Lang UE, Beglinger C, Schweinfurth N, Walter M, Borgwardt S

Because it is found in virtually all living cells, it is often called the "master antioxidant" for its ability to protect the body from oxidative stress

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At 18 h post-treatment, the cells were washed once in mitochondrial assay solution and immediately reconstituted with XF plasma membrane permeabilization buffer supplemented with 5 mM glycerol-3-phosphate and 2 M rotenone