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Desideri E, Ciccarone F, Ciriolo MR

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Following this incubation, the samples were centrifuged again (6,500 g , 5 min), and the resulting pellets were resuspended in a solution of 0.4 N H 2 SO 4 and transferred to a cold room to incubate overnight

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The cancer cells were seeded in a 6-well plate (2 10 5 cells/well) and allowed to incubate for 24 h
