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Based on the manufacturers instructions (GenePharma, Shanghai, China), lentiviral (Lenti- RRM2 shRNA or Lenti-shRNA) (MOI = 15) was applied to transfect TPC-1 and IHH4 cells with 5 g/ml polybrene(Sigma, Cat# H9268) for 12 h

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In the context of rising global obesity rates among women of reproductive age, maternal nutritional status during pregnancy and lactation has become an urgent public health concern

This mtDNA can directly bind to and activate NLRP3, triggering its conformational change and oligomerization [223]

doi: 10.1016/0022-4804(90)90066-B

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