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The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

A unit of enzyme activity is defined as the amount of the enzyme that catalyzes the formation of 1mM of the GSH-CDNB conjugate under conditions of the assay

It often determines which tissue gets the strongest effect

10.1016/j.stem.2012.05.023 107 van den HeuvelM.Harryman-SamosC.KlingensmithJ.PerrimonN.NusseR

did not play a role in the design, conduct, or funding of the study

doi: 10.1038/s41419-020-02871-6